Beschreibung
Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.
Autorenportrait
InhaltsangabeSection I. Semen Analysis Methods 1. Methods For Sperm Concentration Determination Lars Björndahl 2. Methods of Sperm Vitality Assessment Sergey I. Moskovtsev and Clifford L. Librach 3. The Hypoosmotic Swelling Test for Evaluation of Sperm Membrane Integrity Sivakumar Ramu and Rajasingam S. Jeyendran 4. Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow 5. Sperm Morphology Assessment Using Strict (Tygerberg) Criteria Roelof Menkveld 6. Methods For Direct And Indirect Antisperm Antibody Testing Hiroaki Shibahara and Junko Koriyama 7. Manual Methods For Sperm Motility Assessment David Mortimer and Sharon T. Mortimer 8. ComputerAided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation David Mortimer and Sharon T. Mortimer Section II. Tests of Sperm Function 9. The Hemizona Assay For Assessment Of Sperm Function Sergio Oehninger, Mahmood Morshedi, and Daniel Franken 10. The Sperm Penetration Assay for the Assessment of Fertilization Capacity Kathleen Hwang and Dolores J. Lamb 11. Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay Christopher J. De Jonge and Christopher L. R. Barratt Section III. Sperm DNA Damage Testing 12. Sperm DNA Fragmentation Analysis Using the TUNEL Assay Rakesh Sharma, Jayson Masaki, and Ashok Agarwal 13. Sperm DNA Damage Measured by Comet Assay Luke Simon and Douglas T. Carrell 14. Sperm Chromatin Structure Assay (SCSA®) Donald P. Evenson Section IV. Advanced Clinical Testing 15. Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH) Benjamin R. Emery 16. Flow Cytometric Methods For Sperm Assessment Vanesa Robles and Felipe Martínez-Pastor 17. Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions Peter H. Vogt and Ulrike Bender Section V. Sperm Preparation and Selection Techniques 18. Sperm Cryopreservation Methods Tiffany Justice and Greg Christensen 19. Density Gradient Separation Of Sperm For Artificial Insemination David Mortimer and Sharon T. Mortimer 20. Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis Charles H. Muller and Erin R. Pagel 21. Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor Shilo L. Archer and William E. Roudebush 22. Intracytoplasmic Morphology Selected Sperm Injection Luke Simon, Aaron Wilcox, and Douglas T. Carrell 23. Sperm Selection For ICSI Using Annexin V Sonja Grunewald and Uwe Paasch 24. Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh 25. Sperm Selection Based On Electrostatic Charge Luke Simon, Shao-qin Ge, and Douglas T. Carrell 26. Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting Duane L. Garner, K. Michael Evans, and George E. Seidel, Jr. Section VI. Staging and Histology Techniques 27. Assessment of Spermatogenesis Through Staging of Seminiferous Tubules Marvin L. Meistrich and Rex A. Hess 28. Immunohistochemical Approaches for the Study of Spermatogenesis Cathryn A. Hogarth and Michael D. Griswold 29. Ultrastructural Analysis of Testicular Tissue and Semen by Transmission and Scanning Electron Microscopy Hector E. Chemes Section VII. Oxidative Stress Testing 30.
Schlagzeile
InhaltsangabeSection I. Semen Analysis Methods 1. Methods For Sperm Concentration Determination Lars Björndahl 2. Methods of Sperm Vitality Assessment Sergey I. Moskovtsev and Clifford L. Librach 3. The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity Sivakumar Ramu and Rajasingam S. Jeyendran 4. Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow 5. Sperm Morphology Assessment Using Strict (Tygerberg) Criteria Roelof Menkveld 6. Methods For Direct And Indirect Antisperm Antibody Testing Hiroaki Shibahara and Junko Koriyama 7. Manual Methods For Sperm Motility Assessment David Mortimer and Sharon T. Mortimer 8. Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation David Mortimer and Sharon T. Mortimer Section II. Tests of Sperm Function 9. The Hemizona Assay For Assessment Of Sperm Function Sergio Oehninger, Mahmood Morshedi, and Daniel Franken 10. The Sperm Penetration Assay for the Assessment of Fertilization Capacity Kathleen Hwang and Dolores J. Lamb 11. Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay Christopher J. De Jonge and Christopher L. R. Barratt Section III. Sperm DNA Damage Testing 12. Sperm DNA Fragmentation Analysis Using the TUNEL Assay Rakesh Sharma, Jayson Masaki, and Ashok Agarwal 13. Sperm DNA Damage Measured by Comet Assay Luke Simon and Douglas T. Carrell 14. Sperm Chromatin Structure Assay (SCSA®)Donald P. Evenson Section IV. Advanced Clinical Testing 15. Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH)Benjamin R. Emery 16. Flow Cytometric Methods For Sperm Assessment Vanesa Robles and Felipe Martínez-Pastor 17. Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions Peter H. Vogt and Ulrike Bender Section V. Sperm Preparation and Selection Techniques 18. Sperm Cryopreservation Methods Tiffany Justice and Greg Christensen 19. Density Gradient Separation Of Sperm For Artificial Insemination David Mortimer and Sharon T. Mortimer 20. Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis Charles H. Muller and Erin R. Pagel 21. Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor Shilo L. Archer and William E. Roudebush 22. Intracytoplasmic Morphology Selected Sperm Injection Luke Simon, Aaron Wilcox, and Douglas T. Carrell 23. Sperm Selection For ICSI Using Annexin V Sonja Grunewald and Uwe Paasch 24. Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh 25. Sperm Selection Based On Electrostatic Charge Luke Simon, Shao-qin Ge, and Douglas T. Carrell 26. Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting Duane L. Garner, K. Michael Evans, and George E. Seidel, Jr. Section VI. Staging and Histology Techniques 27.
